Part:BBa_K2324004

FimH+SynMT

The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 1 of the mature FimH protein.

This part codes for a FimH adhesin fused with a metallothionein binding domain from Synechococcus elongatus at amino acid residue 1. It is designed to be placed on the end of a type 1 pilus structure in E. coli with a view to binding a number of heavy metal ions. This part is included in the composite part https://parts.igem.org/Part:BBa_K2324009 under the pRha promoter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 879
  Illegal AgeI site found at 306
  
• 1000
  COMPATIBLE WITH RFC[1000]