Regulatory
Part:BBa_K2398003
Designed by: Moritz Przybilla Group: iGEM17_Heidelberg (2017-10-25)
Constitutive Promotor with RFP guide RNA for application in Phage-assisted continous evolution (PACE
This part consists of a gRNA expression cassette for bacteria, flanked by two homology regions for the usage due to the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). Figure one gives a short overview of our standard. Our BioBricks from the registry can easily be used for the assembly of blasmid with the standard (Fig.: 2).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 159
Illegal NheI site found at 182 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
Illegal BglII site found at 223
Illegal XhoI site found at 72 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 146
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |