DNA

Part:BBa_K2289004

Designed by: Sebastian Farias   Group: iGEM17_UChile_Biotec   (2017-10-27)
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STX M30f Aptamer

This part is an Aptamer for Saxitoxin (related to the red tide), this aptamer was developed by Zheng et. al. 2015, and it was selected by a pool of aptamers developed in the same research, this aptamer has a dissociation constant (Kd) of of 0.133 µM. You can see the structure in the absence of the toxin in Figure 1, the conformation of the molecule in the presence of the toxin is still unknown.

Fig.1. Prediction of the conformation of the Aptamer in the absence of the toxin, ublished in Zheng et. al. (2015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

X. Zheng, B. Hu, SX. Gao, D.J. Liu, M.J. Sun, B.H. Jiao, L.H. Wang. (2015). A saxitoxin-binding aptamer whith higher affinity and inhibitory activity optimized by rational site-directed mutagenesis and truncation. Toxicon 101. 41-47.


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Parameters
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