Coding

Part:BBa_K2324001

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-11)
Revision as of 19:52, 1 November 2017 by DanBarber (Talk | contribs)


FimH+sfGFP at residue 225

The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 225 of the mature FimH protein.

CDS which codes for a FimH protein fused with a sfGFP at residue 225 after cleavage of the signal peptide. For characterisation with promoter, RBS and terminator please see https://parts.igem.org/Part:BBa_K2324011

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 185
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None