RNA
Part:BBa_K2486176:Design
Designed by: Guilherme Meira Lima Group: iGEM17_USP-Brazil (2017-11-01)
Synthetic Toehold Switch RNA with GFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 734
Design Notes
The design of this part was inspired by the synthetic toehold switch regulated by a T7 promoter. Since we intended to use this part in another chassis, we decided to replace the T7 promoter with a pLac promoter along with a new terminator.
Source
Adapted from: Green, A., Silver, P., Collins, J., & Yin, P. (n.d.). Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell, 925-939.