Coding

Part:BBa_K2271107:Experience

Designed by: Manuel Lentzen   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 19:49, 1 November 2017 by Manule94 (Talk | contribs)


Applications of BBa_K2271107

Figure 1:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.
Figure 2:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.

The figures 1 and 2 show the functionality of our Pex5 variant R19. R19 with PTS1* leads to clear import whereas the controls with the wild type PTS1 and the wild type Pex5 with PTS1* do not show any signs for import into the peroxisome.

User Reviews

UNIQ4e84dbfed9686c76-partinfo-00000000-QINU UNIQ4e84dbfed9686c76-partinfo-00000001-QINU