Coding

Part:BBa_K2324004

Designed by: Rahan Nazeer   Group: iGEM17_Exeter   (2017-10-12)
Revision as of 19:48, 1 November 2017 by DanBarber (Talk | contribs)


FimH+SynMT

The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 1 of the mature FimH protein.

This part codes for a FimH adhesin fused with a metallothionein binding domain from Synechococcus elongatus at amino acid residue 1. It is designed to be placed on the end of a type 1 pilus structure in E. coli with a view to binding a number of heavy metal ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 879
    Illegal AgeI site found at 306
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None