Coding

Part:BBa_K2271107:Experience

Designed by: Manuel Lentzen   Group: iGEM17_Cologne-Duesseldorf   (2017-10-27)
Revision as of 19:48, 1 November 2017 by Manule94 (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2271107

Figure 1:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.
Figure 2:PEX5 knock out strain, transformed with the R19 variant, mTurqouise tagged with the PTS1 and the PEX13 membrane anchor fused to mRuby.

The figures 1 and 2 show the functionality of our Pex5 variant R19. R19 with PTS1* leads to clear import whereas the controls with the wild type PTS1 and the wild type Pex5 with PTS1* do not show any signs for import into the peroxisome.

User Reviews

UNIQ82bea97735b80895-partinfo-00000000-QINU UNIQ82bea97735b80895-partinfo-00000001-QINU