Coding

Part:BBa_K2194003:Design

Designed by: Catherine Dunaway, Anna Guseva   Group: iGEM17_Rice   (2017-10-23)
Revision as of 19:36, 1 November 2017 by Catdunaway (Talk | contribs) (→‎Source)


cysPUWA Sulfate Transport System cds


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2090
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 3828


Design Notes

[update]

Source

We sourced the nucleotide sequence for cysPUWA from the Escherichia coli MG1655 genome NCBI database (entry NC_000913.3:2539714-2543526). We then sent this sequence to IDT to manufacture as two gBlocks®.

CysPUWA cds NCBI.png

Figure 1: This image is taken from NCBI and shows the cysPUWA genes in their genomic context.

References

[update]