Composite

Part:BBa_K2267040:Design

Designed by: DENG TINGYUE   Group: iGEM17_TUST_China   (2017-10-26)
Revision as of 19:27, 1 November 2017 by Denise Deng (Talk | contribs) (→‎Source)

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LuxR-I-Plux-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 837
    Illegal NheI site found at 860
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 928
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1452
    Illegal BsaI.rc site found at 2179


Design Notes

We characterised the activation range of this device using a GFP reporter. The results of our characterisation experiments can be found here This part does not include an LVA tag on the LuxR gene. This means that the protein is not rapidly degraded. When active, the LuxR protein binds to the pLux promoter, activating transcription of downstream genes. This part contains everything necessary for O3C6-HSL-induced expression of genes inserted downstream of the pLux promoter.

Source

The parts came from the iGEM 2017 DNA distribution.

References