Device
Part:BBa_K2205012
Designed by: Jessica Birt, Declan Kohl Group: iGEM17_Newcastle (2017-09-21)
Sensynova Framework (Blank Processing Module - pCell)
The part BBa_K2205012 was designed as Processor unit of the Sensynova multicellular framework for biosensors development(Figure 1).
In vivo Qualitative Comparison
The connector 1 (C4-HSL) was added to the co-culture consisting of processor + reporter BBa_K2205015 in ratio 1:1. The plot below shows the successful communication via quorum sensing in the Sensynova device. It is clear that the presence of 1mM C4-HSL is detected by the processor cells which produce the connector 2 (C12-RHL) for the reporter cells to detect. This induction in the reporter cells leads to the expression of sfGFP (Figure 2)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 383
Illegal AgeI site found at 580 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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