Composite

Part:BBa_K2232025:Design

Designed by: Wenkai Hu   Group: iGEM17_SZU-China   (2017-10-18)
Revision as of 18:55, 1 November 2017 by James zhang (Talk | contribs) (→‎References)

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mazEF switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 363
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

composite part


Source

Bacillus subtilis

References

  • Considering the biosafety in our project, we used the original part BBa_K302035 from iGEM10_Newcastle team, which is a suicide switch induced by sucrose.
  • Engelberg-Kulka H, Hazan R, Amitai S. mazEF: a chromosomal toxin-antitoxin module that triggers programmed cell death in bacteria[J]. Journal of Cell Science, 2005, 118(19):4327-4332.