RNA

Part:BBa_K2515002

Designed by: Slavil Peykov   Group: iGEM17_Bulgaria   (2017-10-27)
Revision as of 18:46, 1 November 2017 by Slavil (Talk | contribs)


gRNA expression vector for use with Cas9 or dCas9


This is the pSB1C3-gRNA vector designed and used by Team Bulgaria 2017. Our gRNA cloning cassette consist of four functional regions. First comes the constitutive promoter J23119 with defined transcription start. It is fused to the second base pairing region which itself is surrounded by two unique Eco31I sites. Next comes a gRNA scaffold region followed by a S. pyogenes terminator.

For more details and protocols - see Design page.
For a functional test of cloned gRNAs - see Experience page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 64
    Illegal BsaI.rc site found at 38


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Parameters
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