Protein_Domain

Part:BBa_K2348002

Designed by: Andreas Berner   Group: iGEM17_NAWI_Graz   (2017-10-19)
Revision as of 18:42, 1 November 2017 by Andi332 (Talk | contribs)


mCardinal

mCardinal obtained from Addgene [1]
This part was used in our acide induced construct K2348011. In this construct mCardinal is expressed but we were unable to detect any flourescence in E. coli. This might be because, as we later noticed, mCardinal is designed for mammalian expression.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 445
    Illegal BsaI.rc site found at 634


[edit]
Categories
Parameters
None