Composite
Part:BBa_K2232025:Design
Designed by: Wenkai Hu Group: iGEM17_SZU-China (2017-10-18)
mazEF switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 363
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
composite part
Source
Bacillus subtilis
References
Considering the biosafety in our project, we used the original part BBa_K302035 from iGEM10_Newcastle team, which is a suicide switch induced by sucrose.