Part:BBa_K2384004

DsbA+MBP(Hg)

DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .

Our sequence optimization is based on the sequence provided by Peking University's IGEM Wiki in 2010.It is more suitable for expression in Bacillus

Usage and Biology

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.

Before Codon Adjustment

After Codon Adjustment

2.Codon Used Distribution Show the relative codon used distribution

Before Optimization

After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization

After Optimization

The expression of DsbA-MBP(Hg) detected using SDS-PAGE

Plasmid digested by PvuII

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 255
  Illegal BglII site found at 267
  Illegal BglII site found at 711
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]