Composite

Part:BBa_K2384015

Designed by: Fangfei Niu   Group: iGEM17_FAFU-CHINA   (2017-10-27)
Revision as of 17:51, 1 November 2017 by Horace (Talk | contribs)


Repressor gene xylR+Strong PxylA promoter+RBS+Lpp-Omp-MBP(Pb)+His-tag

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Usage and Biology

Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.


Before Codon Adjustment
After Codon Adjustment


2.Codon Used Distribution Show the relative codon used distribution

Before Optimization
After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization
After Optimization
The expression of Lpp-OmpA-MBP detected using SDS-PAGE
Plasmid digested by PvuII
Toggle switch

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None