Regulatory

Part:BBa_K1231000:Experience

Designed by: Viral Patel   Group: iGEM13_Northwestern   (2013-09-13)
Revision as of 17:38, 1 November 2017 by Andi332 (Talk | contribs) (→‎Application NAWI_Graz 2017)


Applications of BBa_K1231000

Application NAWI_Graz 2017

Twin part BBa_K2348001 was created because information in this part was missing. Part itself works and asr promoter is activated at pH 5.0 and 4.5.

User Reviews

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iGEM Dundee 2016

The function of this part was characterised in the hope of designing a pH-responsive system. We hooked up this asr promomoter to an excellent reporter system (BBa_E0840) and characterised its activity under this new composite biobrick (BBa_K1962014). The system showed some pH-dependent activity.

We also hooked up the asr promoter to biobricks encoding immunity proteins towards colicins. This should give some degree of regulation of transcription of the Im-Ia immunity protein (standard biobrick BBa_K1962001 and composite part BBa_K1962015) and the Im-E3 immunity protein (standard biobrick BBa_K1962004 and composite part BBa_K1962016).


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