Coding

Part:BBa_K2448007

Designed by: jeremy armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-10-27)
Revision as of 17:33, 1 November 2017 by Brumake (Talk | contribs)

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PsiR from Sinorhizobium fredii

This part is an E. coli codon optimized version of the PsiR found in Sinorhizobium fredii (NGR_b11520).

Usage and Biology

PsiR is a predicted LacI family transcription factor with high affinity for D-Psicose [1]. This implies that PsiR is potentially capable of binding a consensus sequence in the promoter region and preventing transcription of the regulated promoters in the absence of D-Psicose, in a similar manner to the way LacI does in the absence of allolactose (or the synthetic IPTG).

Our team used this transcription factor together with the Sinorhizobium fredii pPsiA or pPsiR promoters (BBa_K2448012 and BBa_K2448013) to build two Psicose biosensors (BBa_K2448028 and BBa_K2448029). The results show that PsiR behaved as predicted under and without induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 691
    Illegal AgeI site found at 1024
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None