Part:BBa_K2398000

Cytochrome c for the synthesis of organosilicons

We present a codon-optimized version of the cytochrome c protein derived from Rhodotermus marinus. This protein is able to perform the conversion of organosilicons. It is a novel catalytic unit that allows utilization of silicon compounds [Arnold et al., 2016]. When in its mature form, it catalyzes a carbene insertion into silicon-hydrogen bonds.

Usage and Biology

The cytochrome c is used for the catalysis of silicon compounds

Characterisation

Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates

Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates

Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates

Figure 1: Determination of the MIC of beta-lactamases with varying carbenicillin concentration in liquid culture. The OD600 was determined with a sample of 100 µl in 96 well plates

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 93
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]