Measurement
PLac-CI-La
Part:BBa_I763019:Experience
Designed by: Alice Pasini Group: iGEM07_Bologna (2007-08-21)
Revision as of 13:01, 26 October 2007 by Francesca ceroni (Talk | contribs)
We cloned the part inside a high copy number plasmid (pSB1AK3. When we checked part lenght with a gel electrophoresis that was ok. Anyway, when we checked for fluorescence we observed that just some bacteria were fluorescent. We grew bacteria in M9 + lactose + IPTG (2 mM),but always we saw that not all of them were fluorescent.
So, we tried tried the same cloning in a low copy number plasmid (pSB4A3), thinking that a it could better with a lower amount of cI repressor, but we obtained the same result.
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