Coding

Part:BBa_K2317006:Design

Designed by: Shan Wang   Group: iGEM17_Jilin_China   (2017-10-27)
Revision as of 17:06, 1 November 2017 by Eleven (Talk | contribs) (→‎Design Notes)


DmpR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 86
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 564
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 249
    Illegal BsaI.rc site found at 790
    Illegal SapI.rc site found at 1447


Design Notes

2 of the 5 mutation sites are nonsense mutations, just to avoid restriction site.

Source

genebank

References

1.Arlene A. Wise and Cheryl R. Kuske. Generation of novel bacterial regulatory proteins that detect priority pollutant phenols. Appl. Environ. Microb. 2000, 163-169