Coding

Part:BBa_K2381007:Design

Designed by: Anqi Zhou   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 16:39, 1 November 2017 by AnqiChou (Talk | contribs) (→‎Design Notes)

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Rational split dCas9-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 934
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the split method is cutting dCas9 at amino acid site 56 and 714.

Source

The dCas9 is from Streptococcus pyogenes.

References

Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.