Coding

Part:BBa_K2287022

Designed by: Jiang Wu   Group: iGEM17_UCAS   (2017-10-19)
Revision as of 16:18, 1 November 2017 by Wujiang (Talk | contribs)


rhXOR

This is the coding sequence of recombinant human xanthine oxidase which is codon optimized for expression in E. coli. The product of this gene, xanthine oxidase, oxidises hypoxanthine to xanthine and xanthine to uric acid in human purine catabolism. With this enzyme, E. coli is able to transfer xanthine and hypoxanthine into uric acid.（fig.1）

Figure 1: The total pathway of de novo purine biosynthesis with XOR


References

1.M. Ferreira Antunes, F.K. Eggimann, M. Kittelmann, et al. Human xanthine oxidase recombinant in E. coli: A whole cell catalyst for preparative drug metabolite synthesis Journal of Biotechnology., 235 (2016), pp. 3-10 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 612
    Illegal BamHI site found at 707
    Illegal BamHI site found at 848
    Illegal BamHI site found at 1617
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 957
    Illegal NgoMIV site found at 2079
    Illegal AgeI site found at 451
    Illegal AgeI site found at 2458
    Illegal AgeI site found at 2509
    Illegal AgeI site found at 3505
    Illegal AgeI site found at 3859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1438
    Illegal SapI.rc site found at 1474
    Illegal SapI.rc site found at 2071


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