Composite

Part:BBa_K2302010

Designed by: Hongyang Wei   Group: iGEM17_NEFU_China   (2017-10-19)
Revision as of 16:09, 1 November 2017 by Weihongyang (Talk | contribs)

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T7-His-PelB+CALB+T7terminater

This plasmid expresses a fusion protein containing a lipase part and can be transported out the of bacterial membrane. The fusion protein can hydrolyze fat to fatty acids. The expression plasmid consists of four basic parts: the T7 promoter, His, PelB and CALB. PelB is a sequence encoding a functional signal peptide that helps the CALB fusion protein to be transported out of the membrane. CALB is a lipase that can hydrolyze fat. Being transformed with this plasmid, the host bacteria can secrete lipase out the membrane to hydrolyze fat in the environment to fatty acids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1079
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 81
    Illegal NgoMIV site found at 107
    Illegal NgoMIV site found at 800
    Illegal NgoMIV site found at 910
    Illegal AgeI site found at 602
    Illegal AgeI site found at 860
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None