Coding

Part:BBa_K2381006

Designed by: Anqi Zhou   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 16:08, 1 November 2017 by AnqiChou (Talk | contribs)


Rational split dCas9-1

It is a part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a nuclease lobe corresponding to Cas9.

References

Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None