Coding

Part:BBa_K2381007:Design

Designed by: Anqi Zhou   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 15:50, 1 November 2017 by AnqiChou (Talk | contribs) (→‎Source)


Rational split dCas9-2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 934
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the split method is cut it at the loop


Source

The dCas9 is from Streptococcus pyogenes.

References

Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.