Part:BBa_K2379010:Design
Adaptor with FourU RNA thermometer
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1068
Design Notes
The circuit is under the regulation of the pLAC promoter. When inserted in a high copy number plasmid pLAC behaves as a constitutive promoter and hence does not require IPTG induction. However, if inserted in a low copy number plasmid, the system will have to be induced with IPTG as it would then behave like an inducible promoter. GFP, in this case, will be produced only in the presence of IPTG and at 37 degree Celsius.
Source
iGEM Registry
References
1. Konan, K.V. & Yanofsky, C. Rho-Dependent transcription termination in the tna Operon of Escherichia coli: Roles of the boxA Sequence and the rut Site.J. Bacteriol. 182(14), 3981–3988 (2000).
2. Chang, C. L.,Lei Qi, Lucks J.B., Segall-Shapiro T.H., Wang, D.,Mutalik V.K.& Arkin, A.P.An adaptor from translational to transcriptional control enables predictable assembly of complex regulation. Nat. Methods 9,1088–1094(2012).
3. Richardson J.P. Loading Rho to terminate transcription. Cell 114, 157–159 (2003).