Coding

Part:BBa_K2381006:Design

Designed by: Anqi Zhou   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 14:58, 1 November 2017 by AnqiChou (Talk | contribs)


Rational split dCas9-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the split method is cutting at the end of amino acid 57 and the start of amino acid 729. Then connectting them with GSS linker



Source

no

References