Translational_Unit

Part:BBa_K2213003

Designed by: Jessica Burns   Group: iGEM17_Manchester   (2017-08-17)
Revision as of 14:46, 1 November 2017 by Adam hannaford (Talk | contribs)


cgPPK2_His6

Cornybacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal


Usage and Biology





Characterisation


Assessing the thermal stability of cgPPK2 and cgPPK2-mCherry
The heat stability of these constructs was determined through the use of a Thermal Shift Assay of the constructs cgPPK2_His6 (https://parts.igem.org/Part:BBa_K2213003) and PduD(1-20)_cgPPK2_mCherry_His6 (https://parts.igem.org/Part:BBa_K2213004).

Thermalshiftassaymanchesterjess3.png

Firgure 1: Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 761


[edit]
Categories
Parameters
None