Coding
Part:BBa_K2381012
Designed by: Qiqi Xiong Group: iGEM17_HZAU-China (2017-10-20)
Revision as of 14:16, 1 November 2017 by Johnson Huang (Talk | contribs)
3'-OriC gRNA
It’s a gRNA sequence containing 20bp targeting at oriC and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.
The targeting sequence of this part is acquired from xxxx. Its function is proved by OD measurement after cotransformed with plasmid containing dCas9.File:T--HZAU-China--Results Figure1.png. We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.
References
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/basic_part/nominee
None |