Composite

Part:BBa_K2505009:Design

Designed by: Hazuki Hasegawa   Group: iGEM17_TokyoTech   (2017-10-16)
Revision as of 14:06, 1 November 2017 by TakumaY (Talk | contribs)


Ptra-rbs-gfp-tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 821


Design Notes

This part produces GFP induced by 3OC8AHL molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8).

Source

Ptra was derived from agrobacterium.

References

Conserved cis-Acting Promoter Elements Are Required for Density-Dependent Transcription of Agrobacterium tumefaciens Conjugal Transfer Genes, 1995 CLAY FUQUA et.al