Composite

Part:BBa_K2384013

Designed by: Fangfei Niu   Group: iGEM17_FAFU-CHINA   (2017-10-27)
Revision as of 13:54, 1 November 2017 by Horace (Talk | contribs)


Repressor gene xylR+Strong PxylA promoter+RBS+GST-CRS5+His-tag

Xylose-inducible expression system: strong PxylA promoter and repressor gene xylR; after xylose addition, the repressor is released from the PxylA that activates transcription initiation.(BBa_K733002) They have been designed for high expression in B. megaterium This part serves as a functional MT for heavy metal binding and conferred resistance toxic metal cadmium. GST as a fusion partenr is to enhance stability of expression.The original part was from Cornell(BBa_K1460002). This year our parts were doing codon optimisation but without terminator codon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2159
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1571


[edit]
Categories
//awards/composite_part
Parameters
chassis
function