Coding

Part:BBa_K2381001

Designed by: Wenqi Huang   Group: iGEM17_HZAU-China   (2017-10-20)
Revision as of 13:27, 1 November 2017 by Johnson Huang (Talk | contribs)


nMagHigh

nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms.
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts No part name specified with partinfo tag.
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 403


[edit]
Categories
Parameters
None