Coding

Part:BBa_K2201026

Designed by: Christopher M. Whitford   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)
Revision as of 13:23, 1 November 2017 by ChrisMWh (Talk | contribs)


Plasmid for the integration of unnatural base pairs

Plasmid for the retention of unnatural base pairs

Usage and Biology

This part was used as a backbone for the integration of fragments containing unnatural base pairs. The insertion site is located upstream of mRFP, providing an easy screening method. For more on the retention and preservation system, please visit our wiki!

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None