Primer

Part:BBa_K2309031

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-27)
Revision as of 12:22, 1 November 2017 by Zixin.Rong (Talk | contribs)


Standard forward primer for Gibson Assembly to plasmid pNZ8148

This primer contains 18 bp of the standardized biobrick prefix, and a 20-bp-long overhang for Gibson Assembly, which can be digested and ligated into pNZ8148 at the restriction site PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
    Illegal NotI site found at 28
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 22
    Illegal PstI site found at 17
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None