Part:BBa_K2467000
DsbA DspB emGFP
To improve part BBa_K1659211, DsbADspB (dispersin exported to the extracellular using the DsbA signal sequence (KKIWLALAGLVLAFSASA)), we fused it to emGFP in order to measure secreted protein using fluorescence. We amplified the DsbADspB fragment and incorporated EcoR1 and BamH1 restriction sites. The gene was ligated onto the multiple cloning site on the pRSET emGFP vector which resulted in fluorescently tagged DspADspB. This fragment will then be moved to pSB1C3. This allows for easy fluorescence detection in the supernatant contributed by the exported DspADspB. Projects dealing with disruption of Biofilms can easily follow the release of the dispersin enzyme into the extracellular environment to degrade the biofilm polysaccharides
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 316
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 472
| None |