Intermediate

Part:BBa_K2240006:Design

Designed by: iGEM2017_HKUST   Group: iGEM17_Hong_Kong_HKUST   (2017-10-19)
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PluxL with antisense RNA type 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NIL


Source

The promoter was obtained from the iGEM Part Registry while the sequence of the (BBa_K2240002) was obtained from the paper, Development of design rules for reliable antisense RNA behavior in E. coli.


References

Hoynes-O’Connor, A., & Moon, T. S. (2016). Development of design rules for reliable antisense RNA behavior in E. coli. ACS synthetic biology, 5(12), 1441-1454.

Wagner, E. G. H. (2009). Kill the messenger: bacterial antisense RNA promotes mRNA decay. Nature structural & molecular biology, 16(8), 804-806.