Regulatory

Part:BBa_K2447013

Designed by: Chee Wai Kit (david)   Group: iGEM17_NUS_Singapore   (2017-10-05)
Revision as of 11:18, 1 November 2017 by David135 (Talk | contribs)


Temperature-sensitive repressible system

In this construct, the temperature sensitive TlpA36 protein will be produced with the addition of a upstream promoter (as specified by the user), and depending on the temperatures, it will either form Tlpa36 dimers that are capable of repressing the pTlpA promoter (in temperatures below 36 degrees) or stay in monomeric form (above 36 degrees) which makes the protein unable to repress the promoter.

BBA_K2447013 has been characterised using BBa_K2447014 (which is the characterisation construct). Experimental results of this IPTG-inducible temperature system with GFP reporter (BBa_K2447014) had demonstrated that our temperature system is fully functional.

For more information on our experimental result and characterization protocol, do click [http://2017.igem.org/Team:NUS_Singapore/Experiments here].

For more information on how do we improve the overall characterization process by automation, do click [http://2017.igem.org/Team:NUS_Singapore/Measurement here].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 811
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/probiotics/control
Parameters
chassisE.coli (beta 10)