Plasmid

Part:BBa_I732957

Designed by: Zhan Jian   Group: iGEM07_USTC   (2007-10-26)
Revision as of 07:11, 26 October 2007 by ZhanJian (Talk | contribs)

Ptemplate1 + RBS.ARL2A0301.TERM

Genotype Character Name
Top10/pT-TERM So not express any repressors CR00
Top10/pT-ARL4A0604 Constitutively express ARL4A0604 CR01
Top10/pT-ARL2A0203 Constitutively express ARL2A0203 CR10
Top10/pTet-ARL4A0604-ARL2A203 Constitutively express ARL4A0604 and ARL2A0203 CR11

USTC plasmid 957.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1242
    Illegal EcoRI site found at 4409
    Illegal XbaI site found at 4574
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4409
    Illegal SpeI site found at 1243
    Illegal PstI site found at 1257
    Illegal NotI site found at 1250
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4409
    Illegal XhoI site found at 2276
    Illegal XhoI site found at 3302
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1243
    Illegal EcoRI site found at 4409
    Illegal XbaI site found at 4574
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4409
    Illegal XbaI site found at 4574
    Illegal SpeI site found at 1243
    Illegal PstI site found at 1257
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3448
    Illegal BsaI.rc site found at 4433


[edit]
Categories
Parameters
chassis
copies
mcsBioBrick friendly
origin
resistanceAK