Regulatory

Part:BBa_K2448050:Design

Designed by: Jeremy Armetta   Group: iGEM17_Evry_Paris-Saclay   (2017-11-01)
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pPsiTac2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR (BBa_K2448006). A second consensus binding sequence of PsiR was added before the -35 box.


Source

DNA synthesis

References