Protein_Domain

Part:BBa_K2319002:Design

Designed by: Raj Magesh Gauthaman   Group: iGEM17_IISc-Bangalore   (2017-10-24)
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GGSGSGSS linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Serine, like threonine, has a hydroxyl group that contributes to stability by interacting with the aqueous cellular environment. Glycine, again due to its small size, maintains flexibility of the linker.

This linker region also contains a BamHI restriction site (G\GATCC) whose in-frame translation is Gly-Ser. This BamHI site is used to link our two protein domains during assembly of the fusion construct.

Source

This short sequence was added using PCR, with primers having 5'-overhangs corresponding to these nucleotides.

References