Regulatory
Part:BBa_K2194002
Designed by: Catherine Dunaway, Anna Guseva Group: iGEM17_Rice (2017-10-23)
Revision as of 09:57, 1 November 2017 by Catdunaway (Talk | contribs)
PgntK Negative Feedback Membrane Stress Promoter
The PgntK promoter is a native Escherichia coli negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the gntK gene in Escherichia coli, which has been previously experimentally shown to incorporate the full Pgntkpromoter region. [1]
Usage and Biology
[1] Boyarskiy, Sergey et al. “Transcriptional Feedback Regulation of Efflux Protein Expression for Increased Tolerance to and Production of N-Butanol.” Metabolic Engineering 33 (2016): 130–137. Web. 28 Oct. 2017.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 14
Illegal BsaI.rc site found at 216
[edit]
Categories
Parameters
None |