Regulatory

Part:BBa_K2294007

Designed by: Michael Baumschabl   Group: iGEM17_BOKU-Vienna   (2017-10-22)
Revision as of 09:55, 1 November 2017 by Mbaumschabl (Talk | contribs) (→‎Usage and Biology)


Synthtic minimal Galactose induced promoter + Kozak sequence

A short synthtetic promoter which can be induced by the addition of Galactose. This promoter contains already a Kozak sequence for the expression of proteins. The promoter is only 160 bp long.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from GAL1. After this trnaskription factor binding site a neutral spacer is placed to avoid steric hindrances between GAL4 and the TATA-box-binding- proteins of the preinitiation complex.

Promoter characterization

The BOKU-Vienna Team 2017 submitted and characterized this part by measuring the GFP expression using flow cytometry. For detais go to the Experience page.


References

Redden, H., & Alper, H. S. (2015). The development and characterization of synthetic minimal yeast promoters. Nature Communications, 6, 7810. https://doi.org/10.1038/ncomms8810


[edit]
Categories
//chassis/eukaryote/yeast
//direction/forward
//promoter
//regulation/positive
Parameters
positive_regulators