Composite

Part:BBa_K2403002

Designed by: koki yoshimoto   Group: iGEM17_Kyoto   (2017-10-25)
Revision as of 09:51, 1 November 2017 by TongYou (Talk | contribs)


T7 promoter>RRE(Rev Response Element)

You can transport high molecular mRNA into cytoplasm if you can use this parts BBa_2403000. Rev protein binds to RRE(Rev Response Element).


This part contains the RRE (Rev Response Element) of HIV-1 along with a T7 promoter for RNA expression. Together with the HIV-1 Rev protein part (BBa_K2403000), it can be used to efficiently export target RNAs from the eukaryotic nucleus out into the cytoplasm. Details are summarized in this link.

Reference

[1]https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4041468/pdf/gku304.pdf


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None