Coding

Part:BBa_K2309022:Experience

Designed by: Yijing Gong   Group: iGEM17_XJTLU-CHINA   (2017-09-26)
Revision as of 09:42, 1 November 2017 by Zixin.Rong (Talk | contribs) (→‎Applications of BBa_K2309022)


Applications of BBa_K2309022

This year we use dot blot method to evaluate part K2309022 work.

Dot_blot_for_LL-37_XJTLU-CHINA.jpeg

The picture shows the dot blotting result of produced LL-37 which induced by IPTG in BL21 strain. The left one shows the peptide LL-37 produced and right is non-induced BL21 by IPTG.

For LL-37 ability assay, we tested Minimal Inhibitory concentration(MIC) of Staphylococcus aureus, the result is shown as follows:

LL-37_MIC_XJTLU-CHINA.jpeg

As picture shows that the concentration of LL-37 from 5ug/ml to 10ug/ml, the LL-37 has highly activities Staphylococcus aureus.

We also test the ability of LL-37 attact biofilm of Staphylococcus aureus, the result as follows:

LL-37_biofilm_XJTLU-CHINA.jpeg

The result shows that the value of 595nm ABS is dicrease while the increase of LL-37 concentration. It seems that LL-37 peptide inhibite Staphylococcus aureus to form the biofilm.

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