Part:BBa_K2457001
Standardized Cas9 coding sequence + B0015 terminator
This brick was engineered to be a building block of the standard BioBrick vector for one-step bacterial genome engineering: the ([http://2017.igem.org/Team:Amazonas_Brazil pCRISPeasy]). BBa_K2457001 is composed of the Cas9 coding sequence from Streptococcus pyogenes + BBa_B0015 double terminator. It was rationally designed to be interchangeable AND standardized, providing a BioBrick to attend multiple SynBio purposes. For that matter, the RBS and promoter are easily switchable through 3A Assembly. It’s also standard restriction enzymes safe for all RFCs assemblies.
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Usage and Biology
Design
Figure 1: BBa_K2457001 circuit.
Characterization
Figure 2:Sequencing electropherogram from BBa_K2457001.
Figure 3:Alignment of the designed sequence and our final construction from BBa_K2457001.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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