Composite

Part:BBa_K2365052

Designed by: Tong Cheng   Group: iGEM17_NAU-CHINA   (2017-10-18)
Revision as of 09:17, 1 November 2017 by CTosXY (Talk | contribs)


TPI1 promotor-CYC1 terminator

Between the TPI1 promoter and CYC1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 318


We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm

U-disk test.jpg
酵母荧光.jpg
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Parameters
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