Composite

Part:BBa_K2365049

Designed by: Tong Cheng   Group: iGEM17_NAU-CHINA   (2017-10-14)
Revision as of 09:14, 1 November 2017 by CTosXY (Talk | contribs)


PDC1 promoter-CYC1 terminator

Between the PDC1 promoter and cyc1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 289


Pdc1 pdc1-cyc1.jpeg

We inserted GPF (S6GT) as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm

U-disk test.jpg
酵母荧光.jpg
[edit]
Categories
Parameters
None