Part:BBa_K319039:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out. This composite part is based on the Lux quorum-sensing system from Vibrio fischerii. It consists of a Lux receiver device and a GFP reporter that is activated in the presence of a 3O-C6 AHL signal. Quorum sensing is a naturally occurring mechanism that certain strains of bacteria use to regulate gene expression in response to their population density. These bacteria secrete autoinducer signalling molecules, such as N-acyl homoserine lactones (AHLs), that bind to transcription factors to alter gene expression. In this case, the constitutively expressed LuxR transcriptional regulator (C0062) is activated by the binding of 3O-C6 AHL, an AHL quorum signal. The activated LuxR regulator binds to a LuxR-inducible promoter, pLux, upstream of a GFP reporter. As a result, GFP is expressed when the receiver device is induced with 3O-C6 AHL. We designed this part to characterize the activation range of the Lux receiver device.

Applications of BBa_K319039

part1: https://parts.igem.org/Part:BBa_K2267043

part3: https://parts.igem.org/Part:BBa_K2267041

part4: https://parts.igem.org/Part:BBa_K2267042

part6: https://parts.igem.org/Part:BBa_K2267044

part8: https://parts.igem.org/Part:BBa_K2267045

The bacterial population dynamics arising from the synchronized lysis circuit can be conceptualized as a slow build-up of the signalling molecule (AHL) to a threshold level, followed by a lysis event that rapidly prunes the population 。 After lysis, a small number of remaining bacteria begin to produce AHL anew, allowing the ‘integrate and fire’ process to be repeated in a cyclical fashion. We observe growth with the fluorescent protein superfolder GFP (sfGFP). this is the results

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